Pesticides

The Pesticides family includes thirty-eight molecules in the IsoFind catalog, making it the most extensive category. It covers the major classes still detected today in European groundwater and soil, including banned but persistent molecules (atrazine, DDT, lindane) and authorized molecules subject to regulatory monitoring (glyphosate, neonicotinoids, triazoles). This page presents the family structure, applicable regulatory thresholds, tabulated degradation pathways, and available CSIA data.

The Sixteen Sub-families

Pesticides are grouped into sixteen sub-families reflecting standard chemical classification. The most numerous correspond to legacy classes (triazines, organophosphates) and recent classes under increased surveillance (neonicotinoids, chloroacetamides).

Sub-family Count Representative Examples
Triazines 6 Atrazine, simazine, terbutylazine, DEA, DEIA
Organophosphates 5 Chlorpyrifos, diazinon, malathion, fenitrothion
Neonicotinoids 5 Imidacloprid, clothianidin, thiamethoxam, acetamiprid, thiacloprid
Chloroacetamides 4 Metolachlor, S-metolachlor, acetochlor, metazachlor
Phenylureas 3 Diuron, isoproturon, linuron
Organochlorines (incl. HCH & DDT) 4 Dieldrin, endosulfan, DDT, lindane
Phosphonates 2 Glyphosate, AMPA
Triazoles and Fungicides 3 Tebuconazole, propiconazole, tetraconazole
Pyrethroids 2 Cypermethrin, deltamethrin
Others 4 Carbendazim, spinosad, trifluralin, metribuzin

Regulatory Thresholds

Nearly all pesticides in the catalog are governed by two European regulatory frameworks: Directive 98/83/EC (drinking water, individual threshold of 0.10 µg/L) and the Water Framework Directive 2013/39/EU and its updates (AA-EQS environmental quality standards for surface water). The actual threshold applied depends on the specific molecule and the target matrix.

Threshold Category Typical Value Affected Pesticides (Examples)
Dir. 98/83/EC Drinking Water 0.10 µg/L (individual) Atrazine, simazine, diuron, glyphosate, AMPA, terbutylazine
WFD Standard AA-EQS 0.10 to 0.30 µg/L Atrazine (0.60), isoproturon (0.30), diuron (0.20), metolachlor (0.10)
WFD Priority Hazardous AA-EQS 0.005 to 0.030 µg/L Endosulfan (0.005), dieldrin (0.010), DDT (0.025), chlorpyrifos (0.030)
WFD 2022/1441 Neonicotinoids 0.013 µg/L Imidacloprid, clothianidin, thiamethoxam
WFD AA-EQS Pyrethroids 0.0002 µg/L Cypermethrin, deltamethrin
Thresholds vary by several orders of magnitude between a standard pesticide (0.10 µg/L) and a pyrethroid (0.0002 µg/L). Reporting measurements in ng/L rather than µg/L is crucial for the latter: apparent compliance in µg/L can mask an exceedance by a factor of 500 for a pyrethroid. IsoFind normalizes this automatically, but vigilance during data entry remains necessary.

Parent-Metabolite Pairs

Several pesticides in the catalog are accompanied by their metabolites within the same family, allowing for chain-reaction processing. Directive 98/83/EC treats metabolites with the same 0.10 µg/L individual threshold, which simplifies regulatory interpretation.

Parent Metabolites in Catalog
Atrazine Desethyl-atrazine (DEA), desethyl-deisopropyl-atrazine (DEIA)
Terbutylazine Desethyl-terbutylazine
Glyphosate AMPA

Tabulated Degradation Pathways

Ten degradation pathways are tabulated for the pesticide family in the molecule_degradation_pathways database. Each pathway includes its favorable conditions, typical half-life, primary metabolite, and bibliographic reference.

Molecule Pathway Environment t½ (days) Primary Metabolite
Atrazine Biotic Dealkylation Aerobic 75 DEA
Atrazine Chemical Hydrolysis Water-Soil 300 Hydroxyatrazine (HYA)
Atrazine Complete Mineralization (Pseudomonas ADP) Aerobic 15 Cyanuric acid → CO₂ + NH₃
Simazine Biotic Dealkylation Aerobic 90 DEA-simazine
Diuron Biotic N-demethylation Aerobic 90 DCPMU
Diuron Hydrolysis Water 1000 3,4-DCA
Glyphosate Biotic Degradation via AMPA Aerobic 20 AMPA
Glyphosate Degradation via Sarcosine (rare) Aerobic 80 Sarcosine
S-Metolachlor Aerobic Biodegradation Aerobic 50 Metolachlor-ESA
S-Metolachlor Anaerobic Degradation Anaerobic 150 Dechloro-metolachlor
The coexistence of multiple pathways for a single molecule is the rule rather than the exception. Atrazine can degrade through three different pathways with half-lives ranging from 15 to 300 days and distinct metabolites. The dominant pathway depends on local conditions, which the Nexus engine and CSIA bridge handle automatically.

CSIA Data for Pesticides

The catalog contains isotopic fractionation data for six pesticides covering two main elements (carbon and nitrogen) and occasionally chlorine. This data enables CSIA isotopic diagnosis and coupling with the simulation engine.

Molecule Element ε (‰) Range Λ (Coupling) Reference
Atrazine C -5.4 [-6.2 ; -4.5] - Meyer et al., 2009
Atrazine N -2.4 [-3.0 ; -1.8] 0.44 Meyer et al., 2009; Schürner et al.
Simazine C -5.0 [-6.0 ; -4.0] - Meyer et al., 2009
Diuron C -1.8 [-2.5 ; -1.2] - Maier et al., 2013
Diuron N -1.2 [-2.0 ; -0.6] 0.67 Maier et al., 2013
Glyphosate C -2.1 [-2.8 ; -1.5] - Sandy et al., 2020; Adams et al., 2022
Glyphosate N -3.8 [-5.5 ; -2.5] 1.8 Adams et al., 2022
S-Metolachlor C (aerobic) -1.5 [-2.1 ; -0.8] - Alvarez-Zaldivar et al., 2018
S-Metolachlor C (anaerobic) -3.0 [-4.5 ; -2.0] - Droz et al., 2021
S-Metolachlor Cl -5.5 [-7.0 ; -4.0] 0.55 Droz et al., 2021

The coupling coefficient Λ (lambda) represents the expected slope in a dual isotope plot (Δδ element 2 vs. Δδ element 1). It is a powerful mechanistic discriminant: a Λ near 1 suggests control by a bond cleavage involving both elements, while a significantly different Λ suggests a pathway specific to only one of the two elements.

Case Study: Atrazine

Atrazine is the most comprehensive case in the pesticide catalog, with three tabulated degradation pathways, two CSIA elements (C and N), two explicitly recorded metabolites (DEA, DEIA), and extensive regulatory coverage. This richness makes it the reference case for illustrating the possibilities of IsoFind coupling within this family.

Indicator Interpretation
High Atrazine / DEA ratio (>1) Recent input, low degradation or very recent degradation
Atrazine / DEA ratio < 1 Active and advanced degradation, legacy input
Enriched δ¹³C (more positive than source) Confirmed degradation
Dual δ¹³C / δ¹⁵N within Λ≈0.44 Dominant biotic dealkylation
Dual δ¹³C / δ¹⁵N within Λ≈1.13 Dominant abiotic hydrolysis

API Access

Pesticide data is accessible via the molecule module endpoints by filtering for the family. The most useful endpoints for this family are listed below.

Endpoint Usage
GET /api/molecules/reference/catalogue?family=Pesticides List of the 38 reference pesticides
POST /api/molecules/catalogue/seed/Pesticides Pre-fills the project with the pesticides family
GET /api/molecules/csia/Atrazine/pathways Lists tabulated degradation pathways for atrazine
POST /api/molecules/csia/resolve Complete CSIA resolution for a molecule and specific conditions
POST /api/molecules/csia/dual Dual isotope calculation with Λ for mechanistic diagnosis

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